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HighlightsVenom is a liquid or semi-solid substance that is capable of causing harm to living organisms. In terms of the target of venom, it can be generally divided into neurotoxins, blood toxins, cytotoxins, and cardiotoxins. Venom is an extremely dangerous substance that poses a great threat to both humans and animals. Studies have found that anti-venom antibodies are effective in treating venom infections. Based on our hybridoma platform and phage display platform, Creative Biolabs can provide you with custom antibody production services based on your experimental needs. We can provide solutions for different antibodies such as antivenom serum, anti-recombinant antibodies, and single domain antibodies.
The preparation process of venom antigen includes purification of the venom antigen, the combination of small molecular hapten with large molecular weight particulate matter or protein carriers to form complete antigen, and the selection of an appropriate immune adjuvant. Because venom is a composite antigen, it needs to be purified in the preparation of antibodies to obtain the desired single antigenic component. Only a single antigenic component can be used to prepare antibodies with strong specificity. According to the physical and chemical characteristics of the purified venom antigen, one or several appropriate methods can be adopted for purification. The commonly used purification methods include ultracentrifugation, gel electrophoresis, and chromatography.
In the selection of immune animals, it is necessary to select strong, healthy, and age-appropriate animals. The immunity period is generally 45 to 80 days or a little longer, depending on the immunogenicity of the venom antigen, the adjuvant, the method of immunization, and the reactivity of the animal. For the problem of venom antigen detoxification, it is generally determined according to the toxicity of snake venom, protein composition, and others. Usually, the original antigen (the small molecular weight antigen needs to be adsorbed on the macromolecular carrier) combined with an immune adjuvant is injected into the body to prolong the release of antigen and reduce the toxic reaction, and the antigen with a full antibody spectrum and high titer can be obtained. In general, detoxified venom antigens are not necessarily required for the preparation of diagnostic venom antibodies.
The purification of antivenoms is broadly divided into two categories. One is to improve the specificity of antivenoms, and the other is to purify immunoglobulins. There are two main methods to improve the specificity of antivenoms—liquid-phase immunoabsorption and solid-phase immunoabsorption. The liquid phase immunoabsorption method for the purification of specific antibodies from the venom of agitatus has three main steps. In the first step, the cross antigens are detected by AGAR immunodiffusion. In the second step, using the fixed antibody method, the tube with the most complex and the least venom antigen is selected as the equivalent tube, and the venom content of the tube is the amount of venom to be absorbed in 0.05 ml serum. In the third step, the common antibody is prepared and identified and the residual venom is removed.
Creative Biolabs would like to share with you the key points and experiences in the production and application of anti-venom antibodies to facilitate the smooth running of your projects.
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