Human E2F6 Matched Antibody Pair set for ELISA useCapture Antibody: Rabbit purified polyclonal anti-E2F6Detection Antibody: Mouse polyclonal anti-E2F6
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Antigen Detail | |
|---|---|
| Target | E2F6 |
| Description | E2F6 negatively regulates BRCA1 in human cancer cells without methylation of histone H3 on lysine 9. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Polyclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human E2F6 Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-E2F6 Detection Antibody: Mouse polyclonal anti-E2F6 |
| Specificity | Validated for detecting natural and recombinant human E2F6 |
| Research Area | Transcription |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | E2F6 |
| Full Name | E2F Transcription Factor 6 |
| Background | This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. |
| Alternative Names | Transcription Factor E2F6 |
| Gene ID | 1876 |
| Uniprot ID | O75461 |
| HGNC | 3120 |
| Ensembl | ENSG00000169016 |
| OMIM | 602944 |
| Involvement in Disease | Diseases associated with E2F6 include Retinal Cancer and Familial Retinoblastoma. |
| Function | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. |
| Related Pathways | Its related pathways are E2F transcription factor network and Cell cycle. |
For Research Use Only.
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