Aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents, and therapeutic uses. Creative Biolabs makes full use of our specialized libraries, selection strategies, and aptamer maturation techniques to optimize aptamer stability and performance. Specialized techniques are used to select aptamers for specific downstream applications. With advanced technologies and experienced staff, Creative Biolabs provides a comprehensive set of aptamer-related services for global clients to support their research use.
Aptamers are short single-stranded nucleic acids (DNA or RNA) that selectively bind to specific targets including peptides, proteins, and small molecules. Aptamers recognize and bind targets by a 3-dimensional (3D) structure. Since they are chemically synthesized, the process can simply be scaled-up, there are low batch-to-batch variation and high stability. Besides, the production of the aptamer is fast and cost-effective. Aptamers are highly safe biomaterials with no reported in vivo immunogenicity. The specific differences between aptamer and monoclonal antibody (mAb) are shown in the following table.
Table 1 Differences between aptamers and mAb. (Davydova, 2011)
Systematic evolution of ligands by exponential enrichment (SELEX) is a combinatorial chemistry technique for producing aptamers that specifically bind to a target ligand. The technology was first introduced in 1990 and various aptamers have been developed through this method. The aptamers are selected through an iterative process consisting of binding the target with a highly diverse oligonucleotides library, washing unbound aptamer, and amplification of survived oligonucleotides. Multiple rounds of SELEX enhance the enrichment of the oligonucleotides with high specificity for their targets. The best aptamer clones will be selected after sequencing analysis and affinity assay with enriched oligonucleotides.
Fig.1 Schematic illustration of the SELEX process. (Zhuo, 2017)
After the best binding aptamer has been identified, we will send the sequence to the customer. Upon request, we can also ship any remaining aptamer to the customer. This allows unlimited future ordering of additional aptamer from any supplier, with any desired modifications, to be used in any application. With an extensive line of services to support your projects, Creative Biolabs is the best choice for high-quality, 
cost-effective outsourcing/collaborating options for your research programs. We can meet customer’s unmet needs with many kinds of our aptamer services suitable for various aptamer-based assays.
Together Creative Biolabs provides high-value aptamer-related services which include the selection and production of custom optimized RNA and DNA aptamers for analytical, diagnostic, and therapeutic applications. Each aptamer can be provided in various quantities and delivered either unmodified or modified. Creative Biolabs understands that each customer has different requirements for their project and we are happy to work with you to design a selection process to best fit your needs. Any questions about selection service or assay development? Please contact us for more information.
References
For Research Use Only.
