Enzyme-Linked Immunosorbent Assay (ELISA)

Enzyme-linked immunosorbent assays (ELISAs) can be used to detect antigens, antibodies, and haptens. It is a highly sensitive tool in in vitro diagnostics (IVD). Supported by an experienced team of scientists working with the latest molecular diagnosis technology, Creative Biolabs provides a variety of ELISA testing services to meet your testing needs for different samples. We also provide the development services of different types of ELISA kits to support scientific research for customers all over the world.

Introduction of ELISA

ELISA is based on the concept of antigen-antibody reaction. It can directly determine soluble antigens/antibodies in body fluids. In practical application, it can be used for clinical diagnosis, disease surveillance, disease census, forensic examination, veterinary and agricultural plant disease diagnosis, and verification.

ELISA in Newcastle Disease Virus (NDV) Detection

NDV is one of the most severe pathogenic diseases that has detrimentally affected poultry worldwide. Therefore, how to detect the virus quickly is a research topic for scientists. NDV was selected as the antigen by scientists to design the sandwich ELISA using the nanobody as the reagents. Compared with the former assays - the hemagglutination test or hemagglutination inhibition test for detecting NDV particles, the developed sandwich ELISA provides a simple method with high throughput, low-cost, and rapid detection.

Schematic representation of developing the sandwich ELISA to detect antigens using the fenobody and RANbody as reagents.Fig.1 Schematic representation of developing the sandwich ELISA to detect antigens using the fenobody and RANbody as reagents. (Pinpin, 2020)

Our Services

The four main types of ELISAs are direct, indirect, sandwich, and competitive ELISA. Each type of ELISA has its advantages and disadvantages. We provide a growing portfolio of ELISA solutions with high precision, sensitivity and flexibility. Our high-quality services including but not limited to:

  • Direct ELISA Service

The direct ELISA represents the simplest series of steps used to detect antigens/antibodies. We provide direct ELISA testing services to help you save time.

  • Indirect ELISA Service

Indirect ELISA systems have been developed on the basis of direct ELISA. We provide the most suitable strategy to help you amplify the signal and get the most reliable results.

  • Sandwich ELISA Service

A sandwich ELISA measures antigen between two layers of antibodies (capture and detection antibody). The target antigen must contain at least two antigenic sites capable of binding to antibodies. We provide sandwich ELISA detection service with high sensitivity and high specificity for global customers.

  • Competitive ELISA Service

Competitive ELISA is different from direct, indirect, and sandwich ELISAs, as it uses a competitive binding process. We will help you find a suitable detection method according to your project requirements.

  • Development Service of ELISA Kit

In addition to the above ELISA detection services, we also provide customized ELISA kit development services. The antibodies are very important in ELISA kit, which usually determines the sensitivity and specificity of the assay. With years of experience in antibody development, we can help you prepare specific antibodies to develop high-sensitive ELISA kits.

Table.1 Advantages and disadvantages of different types of enzyme-linked immunosorbent assays (ELISAs). (Karishma, 2016)

Table 1. Advantages and disadvantages of different types of enzyme-linked immunosorbent assays (ELISAs).

Creative Biolabs has developed a perfect experimental technology platform for IVD development. Our scientists are proficient in all kinds of enzyme-linked technology, which can effectively ensure the strong stability and high accuracy of our services at a competitive price. If you are interested in our services, you can contact us.

References

  1. Pinpin, J. i.; et al. Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus. Journal of Nanobiotechnology. 2020.
  2. Karishma, Shah.; et al. Enzyme-linked immunosorbent assay (ELISA): the basics. British journal of hospital medicine. 2016.

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