Human Fibronectin antibody pair set for sandwich ELISA useCapture Antibody: Monoclonal IgGDetection Antibody: Biotin-Rabbit Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | Fibronectin |
| Description | Fibronectin is a high molecular weight (~440kDa) glycoprotein in the extracellular matrix that binds to the transmembrane receptor protein integrin. Fibronectin also binds to other extracellular matrix proteins, such as collagen, fibrin, and heparan sulfate proteoglycans (eg, decdecane). Fibronectin exists in the form of a protein dimer, which is composed of two almost identical monomers connected by a pair of disulfide bonds. Fibronectin is produced by a single gene, but the alternative splicing of its pre-mRNA leads to several isoforms. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV01767 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Immunogen | Recombinant Human Fibronectin Protein. |
| Conjugation | Biotin |
| Clone | RD1012 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human Fibronectin antibody pair set for sandwich ELISA use Capture Antibody: Monoclonal IgG Detection Antibody: Biotin-Rabbit Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with Human Fibronectin . |
| Sensitivity | 17.7 pg/mL |
| Range | 62.5 pg/mL-4000 pg/mL |
| Research Area | Cardiovascular; Signal Transduction; Cancer |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | FN1 |
| Full Name | Fibronectin |
| Background | Fibronectin is a high molecular weight (~440kDa) glycoprotein in the extracellular matrix that binds to the transmembrane receptor protein integrin. Fibronectin also binds to other extracellular matrix proteins, such as collagen, fibrin, and heparan sulfate proteoglycans (eg, decdecane). Fibronectin exists in the form of a protein dimer, which is composed of two almost identical monomers connected by a pair of disulfide bonds. Fibronectin is produced by a single gene, but the alternative splicing of its pre-mRNA leads to several isoforms. |
| Alternative Names | FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; fibronectin 1; SMDCF |
| GeneCards | FN1 |
| HGNC | 3778 |
| Ensembl | ENSG00000115414 |
| OMIM | 135600 |
| Involvement in Disease | Diseases associated with FN1 include Spondylometaphyseal Dysplasia, Corner Fracture Type and Glomerulopathy With Fibronectin Deposits 2. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. |
For Research Use Only.
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