Rat EGF Matched Antibody Pair Set [ABP-S-03283] (CAT#: ABP-S-03283)

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This product is an antibody pair set consisting of unlabeled capture antibody as well as unconjugated, HRP or biotin conjugated detection antibody. For some featured antibody pairs, Creative Biolabs also provides standard protein.

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Lot Number

Antigen Detail

TargetEGF
Species ReactivityRat

Capture Antibody

Capture ClonalityPolyclonal
Host SpeciesRabbit
IsotypeIgG
PurificationAffinity purification
BufferPBS, pH 7.4, containing 0.1% Proclin 300
ApplicationELISA; LFIA

Detection Antibody

Det-ClonalityMonoclonal
Host SpeciesMouse
ConjugationBiotin
CloneRD10111
IsotypeIgG
PurificationAffinity purification
BufferPBS, pH7.4, containing 0.09% sodium azide.
ApplicationELISA; WB

General Product Property

Product OverviewThis product is an antibody pair set consisting of unlabeled capture antibody as well as unconjugated, HRP or biotin conjugated detection antibody. For some featured antibody pairs, Creative Biolabs also provides standard protein.
SpecificityBoth the individual capture and the detection antibody in this set react with Rat EGF.
ApplicationELISA; CLIA; ELISPOT; Luminex; ICT
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementAvailable for Research Use Only. Not for use in therapeutic or diagnostic applications.

Target Overview

Gene SymbolEGF
Full NameEpidermal Growth Factor
BackgroundThis gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
Alternative NamesEpidermal Growth Factor; Epidermal Growth Factor (Beta-Urogastrone); Pro-Epidermal Growth Factor; Beta-Urogastrone; HOMG4; URG;
Gene ID1950(Human); 13645(Mouse); 25313(Rat)
Uniprot IDP01133(Human); P01132(Mouse); P07522(Rat)

For Research Use Only.

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ECLElectrochemiluminescence
ELISAEnzyme-Linked Immunosorbent Assay
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ELISPOTEnzyme-linked Immunosorbent Spot
FuncSFunctional Studies
FACSFluorescence-activated cell sorting
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
GSGel Shift
HAHemagglutination Assay
HPLCHigh Performance Liquid Chromatography
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IEMImmuno-Electron Microscopy
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibitInhibition Assay
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RIRadial Immunodiffusion
RIARadioimmunoassay
RIPRadioimmunoprecipitation
StimStimulation Assay
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SDS-PAGESodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
SPRSurface Plasmon Resonance
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