Creative Biolabs provides immune phage display antibody library screening services to generate highly specific antibodies that target common exosome markers and disease-specific markers. These antibodies can be used in the detection, identification, and phenotyping of exosomes, to aid in the study of their morphology and dynamics, and to help elucidate their physiological roles, etc.

Exosome Surface Markers

Exosomes are formed by invagination of the endosomal membrane, thereby generating multivesicular bodies (MVBs). When the MVBs fuse with the plasma membrane, exosomes are released into the extracellular space. They contain mRNA, miRNA, lipids, and a vast array of different proteins depending on their host cell. However they are generally all enriched in proteins involved in MVB formation (e.g., Alix and TSG101), tetraspanins (CD9, CD63, and CD81), membrane transport and fusion (e.g., GTPases, annexins, and flotillins), and a number of cytosolic proteins. These exosomal membrane proteins are currently used to identify exosomes and accepted as exosomal markers.

Fig. 1 Composition and surface markers of exosomes. (Heiler et al. 2016)

Antibody-based Assays for Phenotyping of Exosomes

Antibodies that target exosome markers are widely used in different antibody-based assays for profiling and characterizing the protein composition of exosomes. These antibody-based assays include flow cytometry, western blot, EV (extracellular vesicle) Array, Surface Plasmon Resonance (SPR)-based assays, etc. One advantage of several antibody-based assays is the ability to perform multiplexed phenotyping of exosomes. Multiplexed protein profiling of exosomes provides simultaneous information about multiple biomarkers, holding great potential for delivering data of diagnostic and prognostic value. Moreover, antibody-based assays provide information about features like the cell of origin and the cellular target, which can be translated to hypotheses regarding the functionality of the investigated.

Antibody Screening for Exosome Markers

Constructing and screening immune antibody library via phage display technology is available at Creative Biolabs to produce antibodies with high specificity and affinity against exosome markers. The antibody libraries can reach a diversity of 10^8-10 to meet the needs of the vast majority of research. Our services include:

• Antibody screening for different exosome markers, including exosome-specific markers and disease-specific markers.
• Antibody library screening from various host species, including human, monkey, mouse, rat, rabbit, chicken, camel, etc.
• Antibody validation for different antibody-based assays.

Features of Our Services

• Well-established technology platform for exosome profiling and exosome antibody screening
• A experienced scientient team for supporting the whole-process technical guidance
• Ensured high-quality and low-cost services

Creative Biolabs has years of experience in exosome research. We are confident to offer the best exosome-related services for global customers in an efficient way. In the field of Exosome Antibody Development, we are a world-leading pioneer and offer the complete and high-quality services related exosome antibody. If you are interested in any of our services, contact us and start the conversation.

Reference:

1. Heiler, S.; et al. Pancreatic cancer stem cell markers and exosomes - the incentive push. World Journal of Gastroenterology. 2016, 22(26):5971-6007.

• Premade Phage Display Antibody Library Screening for Exosome
• B Cell Sorting for Exosome Antibody Development