Pediococcus pentosaceus-derived Exosome Research and Application

Pediococcus pentosaceus is a Gram-positive bacterium belonging to the group of lactic acid bacteria, which is one of the common beneficial bacteria in the food fermentation process. In recent years, there has been a gradual increase in exosome-like vesicles of Pediococcus pentosaceus source to regulate inflammatory functions and related studies. Creative Biolabs has accumulated insights into gram-positive bacteria, including Pediococcus pentosaceus, and can provide customized research services on bacterial-derived exosomes.

Isolation of Exosomes from Pediococcus pentosaceus

Culture Pediococcus pentosaceus in medium overnight at 37°C and then collect the supernatant.
Centrifuge at low speed and then filter the Pediococcus pentosaceus supernatant with filters.
After the filter, centrifuge the Pediococcus pentosaceus supernatant several times at ultra-high speed.
Resuspend the centrifuged Pediococcus pentosaceus exosome precipitate with sterile PBS and store frozen.

Studies on Pediococcus pentosaceus-derived Exosomes

Study	Conclusion
Characterization of Pediococcus pentosaceus-derived exosomes.	Compared to E. coli-derived extracellular vesicles, exosomes derived from human intestinal commensals, including Pediococcus pentosaceus, had larger sizes. In addition, disposable capillary cell assay showed that Pediococcus pentosaceus-derived exosomes exhibited high negative electronegativity.
Therapeutic effects of Pediococcus pentosaceus-derived exosomes in liver fibrosis models and vaccination models.	Pediococcus pentosaceus-derived exosomes ameliorated hepatic fibrosis pathology in inflammation-induced mouse models. Pediococcus pentosaceus-derived exosomes negatively regulate vaccine-induced immune responses by inhibiting Th1 cells.
Effects of Pediococcus pentosaceus-derived exosomes on immune cells.	Differentiation of myeloid derived suppressor cells from bone marrow precursors was promoted in mice injected intraperitoneally with Pediococcus pentosaceus-derived exosomes. Treatment with Pediococcus pentosaceus-derived exosomes was capable of inducing myeloid-derived macrophage polarization toward an M2-like immunosuppressive phenotype, inhibiting T-cell activation, and strongly promoting the expression of IL-10, arginase-1, and PD-L1. The results of neutralizing antibody blocking assays showed that this immunomodulatory activity of Pediococcus pentosaceus exosomes was mediated by TLR2.
Effects of Pediococcus pentosaceus-derived exosomes on inflammation-related diseases and wound healing.	Treatment with Pediococcus pentosaceus-derived exosomes reduced the level of neutrophil accumulation in the peritoneal cavity in models of acute peritonitis in mice. Assays of chemical-induced mouse colitis models also showed that Pediococcus pentosaceus-derived exosomes protected against and mitigated inflammation. In mouse skin wound models, intraperitoneal injection of Pediococcus pentosaceus exosomes reduced inflammatory cell infiltration and recruited more MDSC to the wound to favor wound healing.

Fig. 1 Pediococcus pentosaceus-derived exosomes modulate inflammation and therapeutic potential for inflammation-related diseases.¹

It has been shown that Pediococcus pentosaceus-derived exosomes can possess potential therapeutic effects on inflammation-related diseases by modulating inflammation-related signaling pathways, influencing immune cell activity, and improving the balance of intestinal flora, etc. Creative Biolabs has a comprehensive platform for exosome research to facilitate a deeper understanding of Pediococcus pentosaceus-derived exosomes. Please contact us with your project demands.

Reference

Alpdundar Bulut, Esin, et al. "Human gut commensal membrane vesicles modulate inflammation by generating M2-like macrophages and myeloid-derived suppressor cells." The Journal of Immunology 205.10 (2020): 2707-2718.

Plant Exosome Isolation