Phosphorylated Exosomal Proteomic Detection Service

Exosome is a kind of membrane coated nanoparticles or particles, which can protect its internal contents from the influence of external proteases and other enzymes, which provides a new solution for the analysis of phosphorylated proteins. Based on the advanced proteomics analysis platform, Creative Biolabs launches exosome phosphorylation proteomics analysis service.

Background of Protein phosphorylation

Protein phosphorylation is one of the most important and extensive molecular regulatory mechanisms controlling almost all aspects of cell function. The state of phosphorylation events can provide clues about the state of disease. However, few phosphoproteins have been developed as disease markers. Because of the invasive nature of tissue biopsy and the high dynamic of protein phosphorylation in typical long and complex biopsies, the detection of phosphoproteins in tissues is facing great challenges.

Fig.1 Phospho-signaling networks. (Ardito, 2017)

Significance of Proteomic Analysis of Exosomal Phosphorylation

With the discovery of exosomes and more and more studies, the above problems have found solutions. The vesicular properties of exosomes make them highly stable in biological fluids for a long time, and make it possible to develop phosphorylated proteins for medical diagnosis in electric vehicles. The ability to detect genome output (active proteins, especially phosphorylated proteins) can provide more direct and real-time information about biological physiological functions and disease progression, particularly in cancer.

Our Phosphorylation Proteomics Service for Exosome

At present, we have the ability to isolate and identify phosphoproteins from exosomes, and adopt the immobilized metal ion affinity chromatography (IMAC) enrichment strategy to efficiently increase the number of S, T, Y phosphorylated peptides. At the same time, based on Data-Independent Acquisition (DIA) technology, we can collect all signals within the scanning range of mass spectrum without bias, so as to effectively solve the problem of qualitative and quantitative phosphorylation modification.

Fig.2 The workflow for exosomes phosphoproteomics of plasma samples from patients with breast cancer and healthy controls. (Chen, 2017)

Technical Advantages

Full scan mode: DIA mode, greatly reducing the interference of high abundance, comprehensively collecting high and low abundance signals, increasing the data coverage by nearly 40%;
Ultra-high reproducibility and stability: the repetition rate of large sample identification increased by nearly 40%, and the quantitative precision increased by nearly one time;
Ultra-high quantitative accuracy: the quantitative ability is close to the gold standard multiple reaction monitoring (MRM, also known as Selective Reaction Monitoring-SRM) targeting technology.

Creative Biolabs is the research object of exosomes and is committed to providing one-stop service for exosomes research. Through our phosphorylated proteomics analysis service, the development of exosomal phosphoprotein as a potential disease marker is just around the corner. If you are interested in our service, please contact us for more information.

References

Ardito, F., et al. The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy. International journal of molecular medicine. 2017, 40(2): 271-280.
Chen, I. H., et al. Phosphoproteins in extracellular vesicles as candidate markers for breast cancer. Proceedings of the National Academy of Sciences. 2017, 114(12): 3175-3180.

Plant Exosome Isolation