Labeled Exosomal Proteomic Detection Service

Overview Services Features FAQs

Overview

Labeled proteomics involves the use of chemical or metabolic labels to tag proteins or peptides in biological samples before mass spectrometry analysis. These labels can be stable isotopes or fluorescent tags. At present, Creative Biolabs' proteomics analysis platform has successfully realized the labeled proteomics analysis of exosome samples.

Types of Labeled Proteomics

Stable Isotope Labeled:
- Isobaric Tags for Relative and Absolute Quantitation: Uses isobaric tags to label peptides after protein digestion, allowing multiplexed quantification.
Chemical Labeled:
- Tandem Mass Tags: Tags peptides with chemical labels that are identified and quantified during mass spectrometry analysis.

Services

We use isobaric peptide labeling strategies for labeled exosomal proteomics. These strategies primarily target N-terminal and lysine residues (and sometimes cysteine residues) by using N-hydroxysuccinimide reactive groups to facilitate peptide aminolysis, replacing binary adducts composed of mass balance and reporter groups. The different m/z values arise from various isobaric combinations of heavy and light isotopes of carbon (C), nitrogen (N), and oxygen (O) in alternative forms of reagents. These combinations are detected in tandem MS of pooled labeled samples and are used to determine the relative contribution/abundance of peptides, thereby enabling the relative quantification of proteins.

Features

Isobaric tags for relative and absolute quantification technology uses 4-plex or 8-plex isotope labels, while tandem mass tag technology uses 6, 10 and 16 isotope labels, which can simultaneously label 2-16 groups of samples for expression difference analysis;
It is an in vitro labeling technology that can label a variety of samples. It can find membrane proteins, nuclear protein, extracellular proteins, strong base and strong acid proteins, and proteins less than 10 kD and greater than 200 kD. It also has a good detection rate for low-abundance protein;
After mixing different labels, it has good parallelism, avoids technical errors, and improves detection efficiency.

As exosomes are found in more and more samples from different sources, especially some samples with relatively precious and low content, the research on their proteomics has become more and more rigorous. In the proteomic analysis of exosome samples, Creative Biolabs insists on continuously developing methods to improve sensitivity, including introducing new chromatography or MS methods, improving exosome sample preparation methods, etc., so as to better serve exosome research. If you are interested in our service, please contact us for more information.

FAQs

Q: What is the difference between label-free and labeled proteomics, and which one should I choose?

A: Label-free proteomics quantifies proteins without using labels, making it cost-effective and suitable for small sample sizes. Labeled proteomics, on the other hand, involves tagging proteins with labels, providing higher accuracy and quantification in complex samples. If your project involves fewer than 16 samples, label-free might be more economical. For projects requiring high precision and involving more complex sample mixtures, labeled proteomics is recommended.

Q: How much exosome sample do I need to provide for labeled proteomics analysis?

A: For labeled proteomics analysis, please provide at least 400μL of exosome sample. If quantified by particle number, the concentration should be ≥10^10 particles/mL; if quantified by BCA, the concentration should be ≥0.25μg/μL. To ensure reliable and reproducible results, we recommend providing at least three biological replicates per sample.