Protein-Protein Interactions Analysis of Therapeutic Biomolecules

Biological processes almost always involve protein-protein interactions. The interactions of protein-protein are of the utmost importance for molecular biology and drug discovery, as these interactions form the basis of many phenomena. Creative Biolabs has focused on the development of protein design services for many years and has established excellent protein engineering platforms for drug development. We provide a variety of protein design services to meet the diverse needs of our customers.

Introduction to Protein-Protein Interaction

Understanding the function of protein-protein interactions requires knowledge of the structure of the corresponding protein-protein complexes. The purpose of computational protein-protein docking methods is to predict the structure of a protein-protein complex based on the structure of the isolated protein partners. If the structure of the isolated partner proteins is not known, it is often possible to build structures based on sequence homology to a known structure using comparative modeling methods. Currently, there are two types of docking methods for studying protein-protein interactions, one is rigid docking and the other is flexible docking. First of all, receptor and ligand proteins are discretized on three-dimensional grids and are portioned into the inside, surface, and outside regions, respectively. The matching of surfaces is then measured by the overlap of surface regions. For each ligand rotation with respect to the receptor, the correlation problem is solved using Fast-Fourier-Transformation (FFT). After filtering and possible refinement steps, solutions with high overlap of surface regions (high surface complementarity) are collected as putative solutions.

Fig.1 Schematic diagram of the docking process of two proteins.

Services

Protein-protein interaction is fundamental to structural biology and drug discovery. There is an urgent request for computational methods capable of reliably generating and analyzing protein-protein interaction. Rigid docking allows the relative orientations changes of the interacting partners in the modeling, while flexible docking can model changes in the internal geometry of the interacting partners that may occur when a complex is formed. Besides the above two protein-protein interaction analysis methods, we can also provide in silico-based aggregation prediction service to meet customers' specific requirements.

Creative Biolabs has been involved in the field of protein engineering for many years and we are committed to completing your project with high quality. We have accumulated a wealth of scientific experience from our completed projects and provide you with the best services to ensure your requirements are met. If you are interested in our services, please contact us for more details.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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