Creative Biolabs has years of experience in providing custom protein engineering services for global customers. Our services involve rational design and mutant library construction for directed evolution to improve protein stability, affinity kinetics, solubility, activity as well as to discovery novel functions. Additionally, we are specialized in antibody engineering to enhance the clinical efficiency of these promising candidates and reduce their immunogenicity.
Protein engineering based on the use of recombinant DNA technology to change their amino acid sequences to obtain novel or desirable functions. Rational design, which is the most classical method in protein engineering, is an effective approach only when the structure and mechanism of the protein is well-known. In many cases, however, the structure information is quite limited and the mechanism frequently remains unclear. Directed evolution which involves random mutagenesis and selection for the interested protein is a more practical approach. There are many kinds of methods to introduce random or region-specific mutagenesis, such as saturation mutagenesis and DNA shuffling.
Creative Biolabs combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. Based on DNA shuffling method and use of a mixture of restriction endonucleases, we are capable to establish a large library for further screening. We can also generate cell-free translation system, which is based on ribosome display platform for protein engineering and production. Additionally, cell surface libraries coupled with fluorescence activated cell sorting (FACS) or phage display technology are used for efficient high throughput screening.
Figure 1. Protein engineering
Protein engineering is typically performed in vitro, with in vivo consequences and applications. In some cases, Creative Biolabs performs computer modeling and simulations before starting laboratory experiments to make the process of protein engineering more cost-efficient. It includes 3D structure prediction and in silico mutagenesis to predict preferable orientation of binding properties. A variety of parameters will be calculated, such as free energy, affinity kinetics to ensure the accuracy of prediction
Antibody is a specific and large protein that produced by immune cells for therapeutic treatment or diagnostic usage. Antibody-drug-conjugate (ADC) are designed as a target therapy for treatment of people with cancer. Protein engineering are used to enhance the affinity of antibody to target cancer cells or improve antibody stability or reduce its immunogenicity. Additionally, an unnatural amino acid incorporation can significant increase ADC stability through a knobs-into-holes conjugation model. Furthermore, non-antibody scaffolds, such as protein A, a fibronectin domain, an ankyrin consensus repeat domain, and thioredoxin are promising candidates for separation in biotechnology. Some even shows potential pharmaceutical usage in cancer, auto-immuno disease and severe infections. The medical prospects have boosted the antibody and non-antibody scaffolds commercial expectations.
Creative Biolabs has long-term devoted to the development and application of protein engineering. With years of experience, we are capable to provide custom services for protein engineering based on rational design strategy and directed evolution. We are pleased to help you proceed your valuable research with our extensive experience and advanced platform.