IBIS MX96 & CFM System based Epitope Binning Service

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Epitope Binning Services by IBIS MX96 & CFM

An important step in biotherapeutics engineering is to characterize and group (e.g. bin) a library of monoclonal antibodies generated against a specific antigen by their epitope binding regions. Scientists from Creative Biolabs have proved that the use of the IBIS MX96 system in conjunction with the CFM (Continuous Flow Microspotter) offers enhanced productivity in antibody binning.

The IBIS MX96 & CFM combination enables high throughput epitope binning with capacity of 96 antibodies directly from less than 200 µL supernatant of each clone, minimizing sample consumption and overcoming throughput limitations and/or labeling restrictions of current methods. This "epitope binning" platform enables the maintenance of epitope diversity and provides important information to broaden intellectual property protection.

Epitope Binning by IBIS MX96 and CFM

Other optional monoclonal antibody epitope binning services:

FAQ

What is epitope binning, and how is it performed using IBIS MX96 and Continuous Flow Microspotter?
Epitope binning is a process used to categorize monoclonal antibodies based on their binding to specific antigenic sites, known as epitopes, on a target molecule. Using the IBIS MX96, a high-throughput surface plasmon resonance imaging system, and the Continuous Flow Microspotter, which efficiently prints proteins on biosensor surfaces, antibodies can be screened against numerous antigens simultaneously. This combination allows for rapid and detailed analysis of antibody interactions, facilitating the identification of competitive or non-competitive binding patterns among antibodies.
What are the advantages of using IBIS MX96 in conjunction with the Continuous Flow Microspotter for epitope binning?
The primary advantages include high-throughput capability, speed, and precision. The IBIS MX96 can analyze up to 96 interactions simultaneously, significantly speeding up the epitope binning process. When paired with the Continuous Flow Microspotter, which allows for precise and consistent application of antibodies and antigens on the biosensor chip, the system ensures robust reproducibility and scalability in antibody development projects.
Can epitope binning on the IBIS MX96 and Continuous Flow Microspotter be used for all types of antigens?
This system is versatile and can be used for a wide range of antigens, including proteins, peptides, and complex structures like viruses or cells. The flexibility of the Continuous Flow Microspotter in handling different types of biological fluids and the sensitive detection capabilities of the IBIS MX96 make it suitable for diverse applications in immunology, vaccine development, and therapeutic antibody screening.
How does the Continuous Flow Microspotter enhance the specificity of antibody binding during epitope binning?
The Continuous Flow Microspotter enhances specificity by enabling precise and uniform application of antibodies and antigens to the biosensor surface. This precision minimizes cross-reactivity and non-specific binding, which is crucial for accurate epitope binning. The CFM's ability to control flow rates and spot size ensures that each interaction is isolated and well-defined, allowing for clearer differentiation between competitive and non-competitive binding of antibodies to specific epitopes.
How long does an epitope binning experiment typically take using the IBIS MX96 and CFM?
The duration of an epitope binning experiment using the IBIS MX96 and CFM can vary depending on the complexity of the antibody panel and the number of antigens tested. However, typical experiments can be completed within a few hours to a day. This is significantly faster than traditional methods, largely due to the high-throughput nature of the IBIS MX96 and the efficient sample handling by the CFM.