Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsThe epitope of monoclonal antibody correlates with its functional activity. Biacore system is an attractive platform for epitope binning because of its speed and low antigen consumption. With strong expertise and the most advanced Biacore systems in hand, Creative Biolabs is capable of satisfying your demands in antibody binning assays and guarantees you high efficiency and over 99% accuracy.
Biacore is a surface plasmon resonance (SPR) -based detector, which enables the detection of antibody binning in real time without the interference of labels. Using the Biacore system, a single analyte can be injected over up to four serially addressed flow cells, which can be either coated with ligands or used as a reference surface.
Other optional monoclonal antibody epitope binning services:
Fig. 2 Characterization of mouse 1C9 anti-tau2–18 monoclonal antibody. (Michael G. Agadjanyan, 2017)
Previous studies have shown that pathological tau may be a potential therapy for treating advanced AD. The researchers used the hybridoma method to produce mouse monoclonal antibodies and humanized the obtained mouse monoclonal antibodies using CDR grafting. Then, they used HPLC, Biacore, ELISA, IHC, FRET, and other biochemical and immunological methods to characterize and test humanized monoclonal antibodies in vitro/ex vivo/in vivo. Finally, a stable cell line expressing anti-Tau2–18 monoclonal antibody was generated by clone selection.
Epitope binning is a technique used to categorize monoclonal antibodies based on the specific part of an antigen that they recognize, known as the epitope. The Biacore system facilitates epitope binning through surface plasmon resonance (SPR) technology, which measures the interaction between antibodies and antigens in real time. This system allows researchers to determine whether antibodies bind to the same or different epitopes, which is crucial for understanding their mechanisms of action and potential for therapeutic use.
Epitope binning is critical in monoclonal antibody development because it helps identify antibodies with unique binding properties and mechanisms of action. This information is essential for selecting candidate antibodies for therapeutic applications, avoiding cross-reactivity, and designing antibody cocktails that can target multiple epitopes for improved efficacy.
The Biacore system offers several advantages for epitope binning, including high sensitivity, the ability to analyze interactions in real time, and the capacity to measure binding without labeling the antibodies or antigens. This label-free detection preserves the native state of the biomolecules, providing more accurate and relevant biological insights. Additionally, the Biacore system can handle multiple samples simultaneously, increasing throughput and efficiency.
Biacore system can differentiate between competitive and non-competitive binding. By sequentially or simultaneously introducing different antibodies to the same antigen immobilized on the sensor chip, the system can determine if the antibodies bind to overlapping epitopes (competitive) or distinct epitopes (non-competitive). This capability is crucial for mapping antigenic regions and understanding the relationship between different monoclonal antibodies targeting the same molecule.
The Biacore system is equipped with multiplexing capabilities, allowing researchers to analyze interactions between multiple antibodies and an antigen simultaneously. This is facilitated by the system's ability to immobilize different antibodies on separate sensor chip surfaces or different spots on the same chip. The system then tracks the binding kinetics and affinity of each antibody to the antigen in real-time, providing a comprehensive view of the interaction landscape.
Answer: Sample preparation for epitope binning using the Biacore system involves purifying the antibodies and antigens to avoid nonspecific interactions and cross-reactivity. Antibodies and antigens must be solubilized in compatible buffers that maintain their stability and activity. The antigen is typically immobilized on the sensor chip, while antibodies are in the solution phase. The quality and purity of these preparations are critical for obtaining reliable and reproducible results.
Biacore system can be used for epitope binning with small molecule antigens as well as proteins. For small molecules, the system can analyze the interaction between small molecule-targeted antibodies and their antigens, providing insights into the binding specificity and competition with other antibodies. This capability is valuable in the development of therapeutic antibodies against targets where the epitope consists of small molecular structures.
The Biacore system comes equipped with comprehensive data analysis software that assists in the interpretation of SPR data. This software can analyze the kinetics and affinity of antibody-antigen interactions, perform clustering to group antibodies based on their epitope specificity, and visualize the data in various formats, such as sensorgrams and kinetic plots. These tools are essential for accurately determining the binding characteristics of each antibody and facilitating the selection of candidates for further development.
Use the resources in our library to help you understand your options and make critical decisions for your study.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
