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Epitope Binning by ForteBio Octet® Platform

When multiple antibodies have similar affinities for a target, determining whether they bind to the same or different epitopes on the target molecule becomes an inevitable step towards evaluating their individual functional potential. Using our Octet system, Creative biolabs can conduct the epitope binning analysis using samples either purified or directly from hybridoma supernatant or similar matrices.

Octet® system performs sample analysis in 96- and 384-well format, processing up to 16 samples in parallel and requiring only 80 μL-120 μL/well samples in 384 well plates. Antibodies can be assayed in crude mixtures (cell lysates or hybridoma supernatants) or in DMSO (up to 10%) and glycerol, thus drastically reduce the need for sample preparation. The analytical capability of the platform ensures significantly easier, faster and better characterization of antibodies, helping researchers further characterize the specificity of the clones and their ability to block target activity, which can have far-reaching effects on the candidate’s efficacy and pharmacokinetics.

Epitope Binning by ForteBio Octet® platform Fig. 1 Octet data for an array of mAbs binding to Ag that was immobilized in different ways (as indicated in the plot titles). (Abdiche et al. 2012)

Reference

  1. Abdiche, Y. N., Lindquist, K. C., Stone, D. M., Rajpal, A. and Pons, J. (2012) 'Label-free epitope binning assays of monoclonal antibodies enable the identification of antigen heterogeneity', Journal of immunological methods, 382(1), 101-116.

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