This product is a Raji-Luc B cell engineering tool based on the Gold nanoparticles (N-acetyl-D-glucosamine,sialic acid/Wheat germagglutinin) delivery system and CRISPR-based (SHS-229-sgRNA-Cpf1-RS-1 ) genome editor.
| Host Cell | Raji-Luc |
| Target | CCR4 |
| Insert Clone | 14L131 |
| Safe Harbor Sites (SHS) | SHS-229 |
| gRNA | sgRNA |
| Nuclease | Cpf1 |
| Enhancer | RS-1 (RAD51-stimulatory compound 1) |
| Targeting Strategy | pH responsive, time-dependent |
| Insertion sites | Nonhomologous target sites |
| Vehicles | Gold nanoparticles |
| Ligand/Receptor | N-acetyl-D-glucosamine, sialic acid/Wheat germ agglutinin |
| Active Agents | 5-Fluorouracil (5-FU) |
| Particle Size | 1-100 |
| Charge (mv) | −3 to -77 |
| Total Atom Count | ~20,000,000 |
| Surface Atoms (%) | ~4 |
| Specific Surface Area (m2/g) | ~8 |
| Vector Type | Lentivirus |
| Vector Name | BCpCDH2 |
| Targeting Diseases | Breast cancer; Colon cancer; Lung cancer; Ovarian cancer |
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