The many different functions of B cells provide a variety of possibilities for engineered immune cell therapies. Gene-modified primary human B cells, e.g. expressing certain antibody genes, can augment cancer therapy. The generation of such engineered primary B cells can be a challenge requiring extensive cell culture experience and expertise. As a CRO specializing in cell-based immunotherapy development, Creative Biolabs proudly launches the construction service of engineering primary human B cells for R&D work. We have strong expertise to modify primary B cells to express therapeutics, e.g. antibodies with pre-selected specificities.
Flexible & Responsive to your needs
Multiple technology platforms
Technical expertise to tackle complexity
Comprehensive quality assessment
Delivery tool efficiency and safety are of major concern in the genetic engineering of human primary B cells. A series of technologies such as lentivirus, transposons, and CRISPR/Cas9 together with recombinant adeno-associated viral vector (rAAV) have been used to integrate a transgene into the B cell genome. We offer complete B-cell engineering services according to your specifications to help you accelerate your research. You can take advantage of our expertise in gene expression and highly effective gene delivery technologies to produce high-quality engineered B cells.
To overcome the drawbacks of long cycles, low throughput, and low rate of success in primary cell preparation, our team is focused on optimizing strategies and research tools for cell culture and gene manipulation in primary B cells. Our service greatly increases the likelihood of project success — saving you time, effort, and money.
| Services | Deliverables |
|---|---|
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Primary B cell preparation Gene synthesis and delivery Positive cells screening Gene expression evaluation Cell expansion and differentiation |
Engineered B cells Final Report |
Preclinical in vivo therapeutic efficacy assessment of modified B cells in animal models provides valuable information for their later clinical application. We are able to conduct many tests to assess the efficacy of engineered B cells. The technology services our platform offers include:
Fig.1 Workflow of primary human B cell engineering. (Creative Biolabs)
The major objective of our lab is to provide innovative customized solutions for B cell engineering. Our team will handle your R&D service management from the beginning to the end (requires customer consultation to start the project). The turnaround time and price in different B cell engineering projects.
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The following are the results of primary human B cells engineered with α-PD-1 antibody.
Fig.2 A strategy for primary human B cell engineering. (Luo, et al., 2020)
Fig.3 The antibody expression of gene-edited B cells co-cultured with feeder cells. (Luo, et al., 2020)