Selecting a suitable genomic site for the integration of transgene is a key step in B cell engineering. Genomic safe harbor (GSH) loci are well-known safe integration sites without negative effects on the host cell. Therefore, transgenes usually are designed to insert into GSH sites, further improving the safety and efficiency of gene therapy. Several GSH sites have been identified including AAVS1, CCR5, and Rosa26.
The random mechanism of viral-mediated genomic insertions causes safety concerns. While an ideal GSH site doesn't exist yet, CRISPR/Cas9-based GSH method will be more reliable than the random integration of lentivirus or plasmid. At Creative Biolabs, we easily insert a construction into a GSH site of the genome via CRISPR/Cas9 technology. With our well-validated gene targeting strategies combined with our pre-constructed targeting vector materials, our team helps generate precisely targeted, custom B cells.
The common GSH loci include:
In addition to the most advanced gene-editing technology and capacity, we also meet some customers' special needs for random insertion transgene requests.
Creative Biolabs utilizes our pre-constructed vectors or customized vectors to generate gene-modified B cells. The GSH sites are used as docking sites for transgenic constructs. The gene of interest (GOI) can be inserted within the locus so that they are expressed stably. In the presence of a donor vector, homologous recombination (HR) occurs and integrates the GOI knock-in into the GSH locus.
The CRISPR/Cas9 system for B cell engineering commonly includes:
As one of the leading companies in cell therapy, Creative Biolabs has developed a CRISPR/Cas9 platform to engineer B cells including primary B cell and B cell lines. We provide a broad range of solutions to help you create the engineered B cells you need for your research—from culturing cells to gene modification, then analysis and validation.
Get in Touch with Creative Biolabs' B Cell Engineering Services
Yes, customized services are available to meet your specific requirements. Please contact us to design specific gRNAs.
Our solutions enable you to isolate primary B cells and to achieve efficient B cell activation, gene transfer, and expansion.
It depends on the complexity of the project.