UPLC-MS technique is a choice of detection method for the identification of metabolites in biofluids and culture mediums. Creative Biolabs is knowledgeable about the UPLC-TOF-MS analytical technique and B cell biological chemistry. Now we offer the service of metabolic footprinting of B cell to plasma cell differentiation using UPLC-TOF-MS for clients to develop B cell-based therapy.
Metabolic profiling is used to identify and quantity of various molecules in the metabolome to monitor changes in metabolites under different situations. The LC-MS technique is a precious method for an estimate of hundreds of thousands of metabolites in biofluids and tissues. UPLC is a superior chromatography technique for the separation of molecules using a shorter column with decreased size of particles for increased resolution and rapidity. Mass spectrometry can selectively detect and determine the amount of analyte. By hyphenation of the chromatographic system with the spectroscopic system, the performance of MS is largely promoted as the analyte concentration increases after the UPLC process.
With extensive experience in applying UPLC-TOF-MS for the detection and quantity of analytes in a mixed sample, Creative Biolabs offers comprehensive solutions for exometabolic footprinting of B cell to plasma cell differentiation using UPLC-TOF-MS, covering the entire scientific process.
A comparative metabolomics study is performed on various differentiation phases of B cells using the UPLC-TOF-MS technique. Culture supernatant at various time points of the differentiation process is collected for analysis.
A full-scan detection of all metabolites involved in all processes is conducted at Creative Biolabs. The metabolic profile of B cells and plasma cells are identified and analyzed on upregulated and downregulated metabolites.
The collected metabolomic data can be analyzed using principal component analysis (PCA), but not limited to this, and presented in score plots.
When linked right after the UPLC system, the detection profit of MS is appreciably enhanced with improved ionization efficiency and reduced dispersion of chromatography.
Monitor thousands of metabolites in a single sample; analysis of large-scale samples metabolomics.
Integration of data from multiple analytical batches; correction of signals using parallel quality control.
Creative Biolabs is dedicated to providing engineered B-cell differentiation services and characterizing the differentiation process using metabolomics study by NMR and UPLC-FOT-MS techniques. We provide end-to-end solutions and satisfactory results to accelerate your research discovery and development. Please contact us for more detailed information and a quote.