The program of B cell transition into protein production plasma cell is influenced by molecules secreted from surrounding various cells, such as cytokines derived from activated T cells, cellular receptors, and chemokines. These extracellular signals can modify the gene expression landscape and are crucial to deciding cell destiny. Moreover, they are important for plasma cell migration and maintenance in the bone marrow, which is principal for performing a long-lasting function of cells. Based on years of research on plasma cell development mechanisms, Creative Biolabs offers protein-secreting plasma cell generation service by coordinating genome editing and supplementing extracellular signals to facilitate B cell transition toward a plasma cell.
After site-specific modification of B cells for exogenous protein production and secretion, the engineered cells could be differentiated into long-lived plasma cells resident in bone marrow using in vitro and in vivo lineage switch methods. Creative Biolabs' superior expertise can help facilitate your plasma cell differentiation discovery and development studies and provides services covering the entire process of generation and assessment.
As the antibody-secreting cell matures in vivo with T cell help, the engineered B cell can differentiate to protein-secreting cells after transfer into NSG mouse. The differentiation process has two steps.
The differentiation process and composition of the mixed cell cultures can be evaluated using multiple advanced and accurate methods.
Creative Biolabs is devoted to engineering B cells to secret a high capacity of proteins and transit to long-lived plasma cells located in the bone marrow. We are committed to developing the most effective way to guide your engineered B cell differentiation. If you are interested in our service, please contact us and consult our experts.