NMR-based Plasma Cell Metabolite Profiling Service

Plasma Cell Metabolomics and NMR Spectroscopy

During the transit process of engineered B cells to plasma cells, a profound metabolic alteration occurs to accommodate lineage changes to protein-secreting cells. There is not abundant knowledge on the differentiation program, and it is necessary to evaluate the engineered cell products by monitoring the metabolic state of cells during differentiation. Metabolomics allows the global characterization of metabolite parameters, such as compounds consumed and released in the differentiation process, helping to understand the complex process by identifying biomarkers and determining the metabolic pathways in a cell. Nuclear magnetic resonance spectroscopy (NMR) is an advanced, sensitive technique that can be used for metabolomics study.

With cutting-edge techniques and instruments, Creative Biolabs offers plasma cell metabolite profiling by nuclear magnetic resonance spectroscopy (NMR) for clients to study engineered B cell biology.

Services

NMR is robust for the examination of the functional groups and structure of cell metabolic products for metabolomics study. Biological information, such as multiple biomarkers and crucial metabolic pathways, can be acquired from metabolomics allowing us to have a deep analysis of the mechanism of plasma cell construction, function, and phenotype.

Workflow

Main Steps of Our NMR Analysis

• At first, B cells are engineered to synthesize specific proteins and differentiate into plasma cells using appropriate approaches.
• A small amount of cell culture with or without in vitro differentiation is mixed with PBS containing chemical shift references, such as NaN₃, DDS, and TMSP.

• The metabolic profile of plasma cells is applied to the 1D proton NMR spectroscopy technique for analysis.
• Samples are also applied to various types of NMR analysis methods to identify metabolites, but not limited to this.

Data Process Using Advanced Bioinformatics

NMR spectra files are imported and analyzed using innovative analytical technologies and professional biological statistics software. The metabolites are clustered and biomarkers in the B cell activation and plasma cell differentiation process and their effects on a systemic scale are determined.

Highlights

• Perform a complete NMR analysis of your sample
• Provide valuable information for your research and development
• Fast turnaround time for typical analysis
• Affordable cost within your budget
• Expert service in operation and data interpretation
• Complementary analysis of metabolites by LC-MS and elemental analysis

Features and Advantages of NMR Analysis

• Sensitive

Quantify the concentration of metabolites and subtle metabolites in the specimen, and identify impurities and contaminants in your samples.

• Accurate

Determine the chemical structure of organic compounds and phase changes to identify functional groups and conformation changes.

• Multiple valuable data

Discriminate differentiating phases of B cells, deliver metabolic patterns, and identify novel metabolic pathways.

Creative Biolabs is devoted to offering a high-quality NMR analysis on plasma cell metabolite profiling using one-dimensional and two-dimensional NMR to elucidate the structure of components in the mixed culture medium. Our NMR technique service is complete, reproducible, and cost-effective to satisfy your research and development demands. Please contact us and let our experts support your project.

Related Sections:

• UPLC-TOF-MS-based Differentiation Metabolic Footprinting