Cell-intrinsic Gene Regulatory Network based Protein-Secreting Plasma Cell Differentiation Service

Engineered protein-secreting, long-lived plasma cells are potential oncology therapeutics for cancer diseases. With extensive knowledge and program experience in B cell development and differentiation, Creative Biolabs offers protein-secreting plasma cells differentiated from B cells by regulating cell-intrinsic transcriptional factors.

What Is Cell-intrinsic Gene Regulatory Network?

The regulation of protein secretion in engineered B cells is related to the development of plasma cells that differentiate from pre-B cells. Plasmablasts are short-lived, rapid-response effector cells in early antigen-induced response, while plasma cells are long-lasting protein-secreting cells responsible for potential therapeutics. Multiple complex differentiation mechanisms combined with a group of transcription factors control the B cell terminal differentiation to protein-secreting plasma cells. The regulation of gene expression results from the destabilization of several crucial transcription factors associated with B cell differentiation and the expression of plasma-related regulators.

Plasma Cells Generation Services at Creative Biolabs

The ability of B cells to differentiate into long-lived plasma cells, producing and secreting a therapeutic protein, allows the development of innovative plasma cell-based therapeutics delivery to oncology for treatment. With decades years experience and advanced technology, Creative Biolabs offers one-stop solution services for B cell engineering.

Workflow

Fig.1 Workflow of our B cell engineering service. (Creative Biolabs)

• B Cell Sources
  Creative Biolabs provides B cell engineering and differentiation services on primary human B cells, primary mouse B cells, and RAMOS B cells.

• Modulating Regulatory Genes
  B cell terminal differentiation to protein-secreting plasma cells can be realized by modulating the cell-intrinsic gene regulatory network.

Associated Transcription Factors

To induce B cell differentiation to protein-secreting plasma cells, genes associated with B cell identity maintenance are disrupted and drive plasma cell differentiation through the action of transcription factors.

Fig.2 Modulating cell-intrinsic gene regulatory network to induce plasma cell generation. (Creative Biolabs)

Customized Plasma Cell inducing Service:

• You can consult our in-house B cell differentiation expert to choose suitable transcription factors for your research.
• And we can review the literature to find B cell transcriptional regulators of your interest.

Associated Genome Editing Techniques

Creative Biolabs applies multiple genome editing methods to analyze gene roles in B cell differentiation to long-lived protein-secreting plasma cells, including but not limited to below:

• CRISPR/Cas9-based gene disruption
• TALEN-based gene disruption

We have experienced experts in designing gene editing guides targeting each of the above transcription factors with high specificity. Using cutting-edge techniques, we help disrupt target genes independently with high knock-out efficiency in primary B cells.

• Induce Plasma Cell Differentiation in Vitro

Edited B cells are cultured to differentiate into plasma cells in vitro using a well-optimized and validated culture system with multiple crucial and necessary growth factors added in the medium.

Highlights

With a team of Ph.D. leveled experts, Creative Biolabs offers the B cell differentiation service to help clients identify the most proper strategies for the differentiation of engineered primary B cells into long-lived protein-secreting plasma cells. If you are interested in our service, please contact us for a quote.

1. Cheng, R.YH.; et al. Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo. Nat Commun. 2022, 13: 6110.

Related Sections:

• Guiding by Cell-extrinsic Microenvironment