CRISPR/Cas9 system is the latest tool in genome editing. Combining our deep expertise in handling B cells with CRISPR genome editing technology, Creative Biolabs provides researchers with CRISPR-Cas9-based editing methods and comprehensive workflows for B cell engineering—from experimental design to cell line development and validation. We help you design your CRISPR-Cas9 system for precise gene-editing, transfect them efficiently into B cells, and validate the genotypic and phenotypic results. Once the guide RNA and the required vector enter the cells, it can precisely rescript the DNA at the required position to modify the B cell genome in order to express the antibody that is specific for a particular antigen/epitope.
Creative Biolabs offers industry-leading B cells engineering services by CRISPR/Cas9-based genome editing to meet your individual research needs. Our genome editing services are used to precisely insert new genetic material at a defined site, such as the immunoglobulin locus.
Genomic safe harbors are intragenic or intergenic regions of the human genome that enable stable expression of integrated transgenes without negatively affecting the host cell. The CRISPR/Cas9 construct is designed to insert your gene of interest (GOI) into the safe harbor locus for continuous stable expression of GOI. We provide several selections of safe harbor locus including
Creative Biolabs designs CRISPR/Cas9 construct to insert GOI into the immunoglobulin locus. Engineering antibody transgenes by inserting into the immunoglobulin loci can be expressed using B cell endogenous heavy chain constant gene exons.
As an industry leader, Creative Biolabs' dedicated services team focuses on the field of cell and gene therapy. We are using CRISPR/Cas9-based gene editing to create B cells that can stably produce therapeutic antibodies or proteins. Our B cell engineering services are tailored to meet your experimental requirements, helping you to save time and resources.
Fig.5 Workflow of B cells engineering service. (Creative Biolabs)
For more information about our services and capabilities, please contact us.
This is a list of our most frequently asked questions:
We usually perform Western blot or Sanger sequencing. If you have different needs we can discuss them.
Yes, you can contact us to discuss your specific requirements with our experts.
Yes, our services are flexibly tailored according to your requirements.
You will need to provide the cells to be edited, the GOI, the insertion locus, and the validation methods if specified. More details please contact us.
Fig.1 Gene-editing at CCR5 Safe Harbor Locus. (Creative Biolabs) 
Fig.2 Gene-editing at AAVS1 Safe Harbor Locus. (Creative Biolabs) 
Fig.3 Gene-editing at Rosa26 Safe Harbor Locus. (Creative Biolabs) 
Fig.4 Gene-editing at GAPDH Locus. (Creative Biolabs)