Lentiviral vectors play an important role in gene therapy, where genes can be modified, inserted/deleted through the use of lentiviruses. Lentiviruses have the property of becoming endogenous (ERV) and integrating the genes into the host genome, resulting in the virus being henceforth inherited from the host's descendants. Creative Biolabs uses the lentivirus' mechanisms of infection to achieve the desired outcome for B cell engineering for cell therapy.
Fig.1 A kind of lentivirus vector. (Wikipedia)
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Fig.2 Our one-stop solution for B cell engineering. (Creative Biolabs)
Creative Biolabs provides a lentiviral vector-based method to help you engineer B cell for specific cell therapy needs. The following steps are involved in the replication and infection of a lentivirus in a cell:
Step.1 Surface glycoproteins of the virus attach to the outer surface of the cell.
Step.2 Viral material is injected into the cytoplasm of the cell.
Step.3 Viral reverse transcriptase performs reverse transcription of the viral RNA genome.
Step.4 Viral DNA is sent to the nucleus and is integrated into the cell's genome with the help of the viral enzyme integrase.
Step.5 Cells transcribe whole viral RNA and express structural viral proteins.
Step.6 Lentiviral RNA and viral proteins assemble, and newly formed virions leave the cell.
Paper Title: Baboon envelope pseudotyped lentiviral vectors efficiently transduce human B cells and allow active factor IX B cell secretion in vivo in NOD/SCIDγc-/- mice.
Technology: Lentivirus Vector
Journal: Journal of Thrombosis and Haemostasis J Thromb Haemost
IF: 16.031
Published: 2016
Background: B cells are attractive targets for diseases associated with B cell dysfunction, particularly immunotherapy. In addition, B cells are efficient protein-secreting cells and tolerant antigen-presenting cells. This article tests the newly developed baboon envelope (BaEV) pseudotype lentiviral vectors (LVs) for human (h)B cell transduction following adaptive transfer to NOD/SCIDγc-/- (NSG) mice.
Results: BaEV-LVs transduced hB cells under stimulation by B cell receptors are higher than vesicular stomatitis virus G protein VSV-G-LV. BaEV-LV-transduced mature B cells are adoptively transferred to NOD/SCID/γc-/- (NSG) mice, allowing differentiation into plasmablasts and plasma B cells. In addition, the efficient transduction of HB cells and their transfer to NSG mice by BaEV-LVs encoding FIX was evaluated here, demonstrating for the first time that functional hFIX secretion by hB cells at therapeutic levels in vivo.
Lentivirus vector method has been used in many disease therapies:
Fig.3 Application of lentivirus vectors in cell therapy. (Creative Biolabs)
Q1: How to distinguish lentiviral vectors from other viral vectors?
A: Infecting non-dividing cells is a unique ability of lentiviruses.
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