B Cell Engineering Specific Plasmid Products

Product

Filter By Clear All

Construction
Clone
Host
Targeting Cell Type
Vector Type
Species Reactivity
Target

Creative Biolabs offers plasmid design, optimization, and genome integration services based on a novel class of B cell receptors (BCR). We optimize BCRs by modifying extracellular surface tags, transmembrane regions, and intracellular signaling domains, using gene-editing systems such as CRISPR-Cas9 to stably integrate a range of BCR modifications into a variety of vectors. Our B-cell engineering-specific plasmid can achieve several kbs of genome recombination, enables the construction of synthetic BCRs with strong surface expression and antigen recognition, and will be a valuable tool for B-cell immunotherapy.

Nowadays, we focus on plasmid manufacturing, amplification, and purification and can customize the process to your requirements. If you provide a plasmid containing the target sequence as a PCR template, Creative Biolabs will directly construct the vector after receiving the template and sequence. If labels or specific sequences are required, please provide a final version of the final design map or communicate with us. Our automated plasmid DNA preparation platform ensures the stability of plasmid quality between and within batches while enabling high throughput B cell engineering-specific plasmid preparation.

Fig.1 Schematic Representation of The CRISPR-Trap System Plasmid And The Target Region of BCR/ABL Fusion Gene.¹

We also offer different quality control analysis options to meet your needs during the project schedule. Creative Biolabs’ plasmid production system provides more than six QC testing options, aiming to provide customers with high-quality and high-standard B cell engineering-specific plasmid. Routine QC projects include restriction endonuclidene enzyme analysis, residual RNA detection, residual DNA detection, endotoxin analysis, and free target gene sequence sequencing. Moreover, we can provide additional QC service projects such as endotoxin content determination and removal, which can flexibly choose to meet customers' customized needs.

Advantages

Same-day production, fast delivery.
All vectors were Sanger sequenced and the sequences were 100% correct.
All vectors were verified and the experiment was guaranteed.
Complete and systematic quality control standards.

Creative Biolabs is skilled and experienced in B cell engineering specific plasmid production, and has a well-mature GMP-like grade production process, which can provide one-stop services from plasmid construction, cloning and screening, strain construction, process development, non-clinical plasmid production to GMP-like grade plasmid production for pharmaceutical companies and industrial customers. If you are interested in our B-cell engineering products or services, please free to contact us.

Detailed plasmid construction products are as follows.

Reference

Vuelta, Elena, et al. "CRISPR/Cas9-directed gene trap constitutes a selection system for corrected BCR/ABL leukemic cells in CML." International Journal of Molecular Sciences 23.12 (2022): 6386.

RTN4R mAb (mATK63) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR1)