TALEN is a very important gene-editing tool in the treatment of diseases. As a restriction enzyme, TALEN works by engineering to cut specific DNA sequences. In addition to this, TALEN is generated by combining the transcriptional activator-like (TAL) effector DNA-binding domain with the DNA-cutting domain. Based on this excellent genome editing mechanism, Creative Biolabs offers one-stop TALEN gene editing services to help you engineer B cells and bring accurate and reliable data and results to your cell therapy projects.
Fig.1 Drawing of dimeric TALE-FokI fusion bound to DNA. (Wikipedia)
Creative Biolabs provides a comprehensive B cell engineering services to help you achieve your project target. For more T technologies about B cell engineering, please inquire our One-Stop Solution for B Cell Engineering.
Fig.2 Our one-stop solution for B cell engineering. (Creative Biolabs)
Creative Biolabs provides an all-in-one TALEN solution for B-cell knockout/knock-in genome editing to accelerate your project. More details of the TALEN workflow are below:
Step 1 Design the TALEN sequence.
Step 2 Insert it into the plasmid.
Step 3 Insert the plasmid into the target cells (generate functional TALEN).
Step 4 TALEN enters the nucleus (bind and cleave the target sequence).
Step 5 Erroneous or new DNA sequences can be introduced into the target gene (Depending on the application).
Fig.3 Workflow for genome editing using TALEN. (Wikipedia)
Paper Title: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Technology: TALEN-based genome editing
Journal: Nucleic Acids Res
IF: 19.16
Published: 2011
Background: Fusion of transcriptional activator-like (TAL) effectors with FokI nucleases in plant pathogenic Xanthomonas species. TALENs bind in pairs and cut DNA. Binding specificity is determined by amino acid repeats in TAL effectors. This article establishes a method for efficiently assembling TALEN constructs. In addition, it describes design guidelines based on naturally occurring TAL effectors and binding sites.
Results: This article shows that TALENs targeted with the software previously described by the scientists and constructed using plasmid sets are active in yeast DNA cleavage assays. Besides, it is also effective in gene targeting in human cells and Arabidopsis thaliana protoplasts. In addition, a functional analogue of the Xanthomonas avrHah1 TAL effector gene was successfully constructed.
TALEN is useful to manipulate the genomes of many species:
Fig.4 Applications of TALEN in different species. (Creative Biolabs)
Q1: What methods can be used for engineering TALEN constructs?
A: One solution is a publicly available software program (DNAWorks). The other solutions include modular assembly schemes.
We offer professional consultation guidance before, during and after the project. For more information on TALEN, please contact us for your tailored solution.