B Cell Engineering Service for Therapeutic B Cell Activating Factor (BAFF) Delivery

Exploiting B Cell Edited with Cell Activating Factor (BAFF) for Cancer Therapy

BAFF is a TNF-like cytokine that supports the survival and differentiation of B cells. Studies have shown that B cells engineered to express hBAFF exhibit higher expression of CD38 and IgG, and increased proliferation. Scientists at Creative Biolabs are glad to apply our special expertise in engineering B cells with BAFF to help our worldwide customers with their cutting-edge research projects.

Your Project Will Benefit from Our Extensive Expertise

Human plasma cells are a terminally differentiated B cell population that resides in the bone marrow and stably produces high concentrations of antibodies. This long-lived plasma cell (PC) is an alternative method to express and deliver protein drugs. Organizing a staff of outstanding scientists who have engaged in the research of B cell engineering for many years, Creative Biolabs is proficient at engineering human B cells to express BAFF, differentiated them into PCs, exhibiting crucial advantages for delivery.

Workflow of B Cell Engineering Service at Creative Biolabs

Fig.1 Workflow of B cell engineering. (Creative Biolabs)

Advantages

• Human long-lived plasma cells (PCs)
• Precise dose-control
• Quiescence in the cell product,
• Low risk of mutation in the sequence of therapeutic protein

Editing B Cell at Safe Harbor Loci

Engineered B Cells Assessment Service

• In Vivo Survival Assessment Service
  We assess cell expansion and plasmablast formation following genome engineering and in vitro differentiation. In the BAFF-edited plasma cells, the increase of cell number, viability, and percentage of CD19lowCD38high plasmablasts can be observed. After the generation of gene-edited B cells, immunodeficient NOD/SCID/gamma-c null (NSG) mice will be used for survival assessment. Edited cells will be differentiated into plasma cells in vitro and adoptively transferred into NSG mice via intravenous infusion. We are experts in survival assessment in vivo. Write us to know more, we are looking forward to hearing from you.
• Functional Assessment Service
  In order to assess the functionality of engineered B cells, fluorescence-activated cell sorting (FACS) will be applied.

If it is a therapeutic antibody to be engineered, whether it is a functional monoclonal antibody or not will be assessed by FACS.

Creative Biolabs is well-equipped and versed in engineering B cells for BAFF delivery. With proven experience in this field, Creative Biolabs is an ideal company to entrust with your business. Please feel free to contact us for more information.

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