As natural antibody-secreting cells, B cells have several advantages for therapeutic delivery. Engineering B cells opens a new avenue to treat various diseases and to study their basic biology. Since the huge potential of the engineered B cell/plasma cell lineage for therapeutic applications, Creative Biolabs is dedicated to developing B cell-based therapy. We have developed optimized strategies to reprogram primary mouse B cells to generate therapeutic antibodies and proteins. Effective transfer and expression of the gene of interest (GOI) is the key step in B cell engineering. Our gene-editing platform and delivery system confers high efficiency of gene transfer into primary mouse B cells, promoting the development of B cell-based therapy.
Targeted modification of a cell's DNA via genome editing provides a powerful tool for the development of novel cellular therapeutics. Creative Biolabs focuses on the field of cell and gene therapy. Now, our team is dedicated to helping our clients modify primary mouse B cells using the most robust and reliable technologies on the market. Equipped with state-of-the-art technologies, scientists at our lab provide several available strategies for the safe modification of primary mouse B cells. The following are several technologies we provide.
CRISPR/Cas9-based Genome Editing Service
CRISPR/Cas9 is an RNA-guided genome editing tool to create targeted genomic modifications in cell lines and animals.
TALEN-based Genome Editing Service
TALENs is a protein-based DNA targeting system. We use TALEN-mediated cell engineering to produce gene-modified B cells.
Homology-directed Repair (HDR) Technology
HDR technology uses homologous donor DNA to repair DNA damage. It holds great potential in gene therapy since it allows precise customization of the genome.
Adeno-associated Viruses (AAV) Vector
AAV is a common vector for gene transfer. We create gene transfer vectors derived from AAV for the transduction of primary mouse B cells.
B Cell Engineering by Lentivirus Vector
Lentivirus vectors are promising tools to efficiently transduce gene of interest into primary B cells. Generally, lentiviral vectors are efficient for long-term expression.
B-cell engineering is a critical and potentially time-intensive step in the development of cell and gene therapy. Our scientists have the ability to successfully initiate and complete critical phases of B cell engineering. We offer choices in technologies and services to support your research and clinic translational needs.
These technologies vary from efficiency, price, genomic location of transgene integration, and specificity. Our experienced team of scientists will work with you to choose the best development method for your specific requirements.
We perform Sanger sequencing as well as evaluating protein expression via Western Blot.