Since B cells and plasma cells can support antitumor immune responses through several mechanisms, as shown in Fig.1. B cell engineering has been a valuable technology in the area of immunology therapy. Technically, B cells engineering is complicated and challenging, because they could bind their target antigen only after the engineering cassette is successfully inserted into the right locus. Even though, fully functional B cells still require post-translational modifications to produce membrane-bound and secreted antibodies. To fulfill this imperative need, Creative Biolabs offers the service of target antigen quantitation of B cells for the functional check.
We offer scientific and meticulous service for B cell target antigen quantitation, mainly including flow cytometry assay (FC), enzyme-linked immunosorbent assay (ELISA), and data analysis to ensure high-quality B cell engineering results. For more information about the assessment of engineered B cells functionally in vivo and in vitro, please inquire about our Engineered B Cells Assessment Service.
Fig.1 Our target antigen quantitation service. (Creative Biolabs)
Creative Biolabs is committed to efficiency, confidentiality, and rapid, accurate service with proven experience and expertise.
Fig.2 Our features and benefits. (Creative Biolabs)
Paper Title: B cells engineered to express pathogen-specific antibodies protect against infection
Technology: CRISPR/Cas9; Target Antigen Quantitation
Subjects: RAMOS B cells and Primary human B cells
Journal: Science Immunology
Published: 2019
Background: Effective vaccines inducing lifelong protection against respiratory syncytial virus (RSV) are not yet available despite decades of research.
Results: B cells engineered to express an antibody against RSV resulted in potent and durable protection in RAG1-deficient mice.
Q: When the target antigen quantitation assay is needed?
A: Pre-experiments to test the feasibility of B cell engineering strategies; Evaluation of the strategy optimization effect of B cell engineering; Assessment of the antibody production level by engineered B cells in vitro and in vivo; Validation of the protective effect of animals from viral infection after transplantation of engineered B cells.
Q: What's the difference between FC and ELISA, two different target antigen quantitation assays?
A: FC could detect membrane-bound antibodies while ELISA could detect the secreted antibodies in the supernatant of cell culture or the serum of animals. Fully functional B cells could produce both membrane-bound and secreted antibodies.
Creative Biolabs offers professional consultation during the project. Please contact us for personalized solutions based on your specific needs.