Engineering B Cell Types for Long-Lasting Treatment Applications

Services

B cells present a promising candidate for novel cell-based therapies because of their ability to secrete large amounts of protein and persist for the life of the organism. In theory, plasma B cells can survive decades—even the entire life of the patient. Some research has shown that engineered human antibody-secreting B cells can survive for greater than a year in a living model. With highly innovative, next-generation enabling technologies under one roof and numerous experts in each field, Creative Biolabs has built a powerful engineering B cell platform for therapeutics delivery, opening a new and paradigm-shifting class of cellular medicines. Through precision engineering, we genetically engineer B cells to act as cell factories capable of delivering continual, high doses of a therapeutic protein.

Fig.1 A schematic of the conceptual framework of B cell engineering for therapeutic protein secretion. (Hung, et al., 2018)

B Cell Engineering Services

Primary Human B Cell Engineering Service

With extensive experience in isolating and culturing primary cells, Creative Biolabs provides primary B cell isolation from PBMC cells or blood samples. We are dedicated to the custom development and engineering of primary B cells to produce biomolecules, such as therapeutic proteins and antibodies.

RAMOS B Cell Engineering Servic

RAMOS B cell is a human Burkitts Lymphoma cell line that offers several advantages over primary B-cells. The cells have B lymphocyte characteristics, with surface-associated mu and kappa chains. At Creative Biolabs, we use technologies such as the CRISPR/Cas9 method to rapidly generate engineered Ramos cell lines.

Primary Mouse B Cell Engineering Service

Creative Biolabs has optimized transfection and culture conditions for primary B cell gene modification. We provide flexibly tailored engineering solutions for primary mouse B cells isolated from single-cell suspensions of splenocytes or other tissues.

Therapeutic Focus

Recent developments in gene manipulation and a deep understanding of cell-based therapy have opened new possibilities for B cell gene therapy. Creative Biolabs has developed methods for ex vivo expansion and targeted genome engineering of primary human/mouse B cells. Our genetically-engineered B cells confer new specificities to B cells and open a new avenue for using B cells to treat various diseases and to study their basic biology. Engineered B cells can be used in the treatment of multiple diseases, including:

Infectious diseases
Hematological tumors and solid tumors
Autoimmune diseases

Creative Biolabs has a research team of staff with expertise in hematology, oncology, neurology, immunology, molecular medicine, and immune therapy to develop new cancer treatments. Based on our B cell engineering platform, We are accelerating the future of B cell therapy with flexible solutions that manufacture healthier and more functional B cells. If you are interested in our B cell engineering services, please feel free to contact us to discuss more.

Frequently Asked Questions

What Quality Control (QC) tests do engineered B cell lines undergo?

Bacterial and Mycoplasma testing
Cell count & viability

Can I use my own B cells for engineering?

Yes, you can send your cells to us. We will confirm that the cells are mycoplasma free at first.

How many cells do you deliver?

We commonly provide 1-2 vials of engineered B cells. Each vial of cells typically contains 1 million cells.

Contact Us

We perform various strategies to generate custom B cells. Tell us your research needs Creative Biolabs offers a complete solution for your project.

Reference

Hung, K.L.; et al. Engineering protein-secreting plasma cells by homology-directed repair in primary human B cells. Molecular Therapy. 2018, 26(2): 456-67.