B cell engineering can serve as a novel strategy for immunotherapy. Engineered B cells expressing the gene of the therapeutic antibody can function as both BCRs and subsequently as secreted antibodies from long-lived plasma cells. Therefore, B cells can serve as a platform for cell-based therapy. Engineered B cells express the desired Ig and disrupt endogenous Ig production by insertion of antibody transgenes into the immunoglobulin loci of B cells.
Fig.1 Covalent Attachment of ssODN to PCV Fusions of Cas9 Enhances Insertion of a Peptide Tag.1,2
Creative Biolabs has developed efficient strategies to gene edit human and mouse B cells. B cell engineering service is performed by CRISPR/CAS9 gene-editing technology to help researchers insert their gene of interest in the specific genomic locus through homologous recombination. This technology enables precise targeting of the immunoglobulin loci and enables the insertion of virtually any sequence to produce an antibody while at the same time silencing the expression of the endogenous antibody genes.
Creative Biolabs offers custom services for designing, creating, and validating gene-modified B cells. The following are several published strategies for B cell engineering by gene editing at the immunoglobulin loci.
The CRISPR/Cas9 delivery methods involve both the vehicle and cargo.
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Creative Biolabs has developed a series of strategies to help clients insert genes of interest in a genomic locus through CRISPR/CAS9 technology. Precise modification is performed by transfection into target cells with gRNA and Cas9 nuclease. We provide design, synthesis, and validation services for guide RNA.
The specific genomic loci for gene integration include:
B cell engineering to express therapeutic antibodies is a safe, potent, and scalable method. It has been widely applied for various study goals and disease purposes:
Q1: Can I send my own cells for engineering?
A1: Yes, this is possible. If you wish to use your own cells, please contact us and our team will perform engineering service on the indicated B cells on your behalf.
Q2: What guarantees are in place for your engineered B cells?
A2: We guarantee the engineered cells are mycoplasma free and validated at the genomic level by Sanger sequencing.
Q3: Can you provide an off-target analysis report?
A3: Yes, upon the customer's request.
Q4: What are the deliverables?
A4: Genetically engineered B cells and final report.