This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-hPGK-Sec/SPII-CD79α-CD28-Myc) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-HD/Cas10-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | HA22SHW |
| Host | Mouse |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | RFP |
| Promoter | hPGK |
| Signal peptides | Sec/SPII |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | GSAT Linker |
| Co-Receptors | FcγRIIB |
| Vector Type | MMLV |
| Vector Name | BGpCDH5 |
| Class | 1 multi-Cas |
| Type | VI |
| Subtype | A, B, C |
| Effector | Cas12a (Cpf1) |
| DNA Type | dsDNA |
| Nuclease domains | HD/Cas10 |
| TracrRNA requirement | YES |
| PAM/PFS | NNGRRT |
| Insertion | Wap |
| Construction | RFP-hPGK-CD79α-Cas12a (Cpf1)-dsDNA-Wap |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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