This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-mPGK-Sec/SPII-CD79β-CD8-Myc) and CRISPR-Cas (Cas13c (C2c7)-RNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | HITK22 |
| Host | Mouse |
| Targeting Cell Type | Toledo |
| Reporter gene | CAT |
| Promoter | mPGK |
| Signal peptides | Sec/SPII |
| Signaling domains | CD79β |
| TM domains | CD8 |
| Detection tags | Myc |
| Linkers | Thioredoxin with polylinker |
| Co-Receptors | CD22 |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 1 multi-Cas |
| Type | III |
| Subtype | A, B, C |
| Effector | Cas13c (C2c7) |
| DNA Type | RNA |
| Nuclease domains | HD/Cas3 |
| TracrRNA requirement | YES |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | CAT-mPGK-CD79β-Cas13c (C2c7)-RNA-Stat5b |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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