This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-EF1A-Sec/SPIII-CD79β-CD28-Flag) and CRISPR-Cas (NmCas9-dsDNA-2xHEPN-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | SP1P9 |
| Host | Rabbit |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | RFP |
| Promoter | EF1A |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | Flag |
| Linkers | Glycine linker |
| Co-Receptors | LILRB |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 2 single-Cas |
| Type | IV |
| Subtype | D |
| Effector | NmCas9 |
| DNA Type | dsDNA |
| Nuclease domains | 2xHEPN |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | attP |
| Construction | RFP-EF1A-CD79β-NmCas9-dsDNA-attP |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.