This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-MNDU3-Sec/SPIII-CD79β-CD28-HA) and CRISPR-Cas (Cas12a (Cpf1)-ssRNA-RuvC, HNH-5' PFS: non-C; 3' PFS:NAN/NNA-Stat5b) systems.
| Clone | CUXAb1 |
| Host | Mouse |
| Targeting Cell Type | P3HR-1 |
| Reporter gene | GUS |
| Promoter | MNDU3 |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | HA |
| Linkers | Short Flexible Linker |
| Co-Receptors | LILRB |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 1 multi-Cas |
| Type | VI |
| Subtype | A, B, C |
| Effector | Cas12a (Cpf1) |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | 5' PFS: non-C; 3' PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GUS-MNDU3-CD79β-Cas12a (Cpf1)-ssRNA-Stat5b |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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