This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (lacZ-MNDU3-Sec/SPIII-CD79β-CD8-Myc) and CRISPR-Cas (SaCas9-dsDNA-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | BGP-0969 |
| Host | Mouse |
| Targeting Cell Type | Raji |
| Reporter gene | lacZ |
| Promoter | MNDU3 |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79β |
| TM domains | CD8 |
| Detection tags | Myc |
| Linkers | Z-EGFR-1907 Linker |
| Co-Receptors | LILRB |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 2 single-Cas |
| Type | V |
| Subtype | A, B, C, D, E, F, U |
| Effector | SaCas9 |
| DNA Type | dsDNA |
| Nuclease domains | Unknown |
| TracrRNA requirement | YES |
| PAM/PFS | 5' AT-rich PAM |
| Insertion | ROSA26 |
| Construction | lacZ-MNDU3-CD79β-SaCas9-dsDNA-ROSA26 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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