This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-SV40-Sec/SPI-CD79α-CD28-HA) and CRISPR-Cas (Cas12b (C2c1)-RNA-RuvC, HNH-NNNNGATT-Alb) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | IR-26126 |
| Host | Mouse |
| Targeting Cell Type | Jiyoye [Jijoye, P-2003, P-3J] |
| Reporter gene | GUS |
| Promoter | SV40 |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | HA |
| Linkers | Glycine-Serine linker |
| Co-Receptors | LILRB |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 1 multi-Cas |
| Type | IV |
| Subtype | A, B |
| Effector | Cas12b (C2c1) |
| DNA Type | RNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | Yes |
| PAM/PFS | NNNNGATT |
| Insertion | Alb |
| Construction | GUS-SV40-CD79α-Cas12b (C2c1)-RNA-Alb |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.