This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-CMV-Tat/SPI-CD79α-CD28-Flag) and CRISPR-Cas (SaCas9-ssRNA-RuvC, Nuc-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | 9UL7 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | RFP |
| Promoter | CMV |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Flag |
| Linkers | Z-EGFR-1907 Linker |
| Co-Receptors | Siglec-G |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 2 single-Cas |
| Type | Ⅴ |
| Subtype | A, B, C |
| Effector | SaCas9 |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, Nuc |
| TracrRNA requirement | No |
| PAM/PFS | - |
| Insertion | Stat5a |
| Construction | RFP-CMV-CD79α-SaCas9-ssRNA-Stat5a |
| Target | FOLR1 |
| Alternative Names | FOLR1; FBP; FOLR; Folate receptor 1; folate receptor 1 (adult); folate receptor alpha; FRalpha; NCFTD |
| Gene ID | 2348(Human); 171049 Rat(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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