This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-CBh-Tat/SPI-CD79α-CD28-CBP) and CRISPR-Cas (NmCas9-RNA-RuvC, HNH-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | RA15-7 |
| Host | Rat |
| Targeting Cell Type | Raji-Luc |
| Reporter gene | Luc |
| Promoter | CBh |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | CBP |
| Linkers | G4S linker |
| Co-Receptors | CD22 |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 1 multi-Cas |
| Type | IV |
| Subtype | A |
| Effector | NmCas9 |
| DNA Type | RNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | YES |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | Luc-CBh-CD79α-NmCas9-RNA-Stat5b |
| Target | FOLR1 |
| Alternative Names | FOLR1; FBP; FOLR; Folate receptor 1; folate receptor 1 (adult); folate receptor alpha; FRalpha; NCFTD |
| Gene ID | 2348(Human); 171049 Rat(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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