This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-CBA-Tat/SPI-CD79α-CD8-His) and CRISPR-Cas (SaCas9-ssRNA-RuvC-NGG-Csn) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | 5OI4-2RT9 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | YFP |
| Promoter | CBA |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | His |
| Linkers | (SSSSG)x2 serine glycine linker |
| Co-Receptors | CD81 |
| Vector Type | Lentivirus |
| Vector Name | BGpCDH2 |
| Class | 2 single-Cas |
| Type | Ⅴ |
| Subtype | A, B, C, D, E, F, U |
| Effector | SaCas9 |
| DNA Type | ssRNA |
| Nuclease domains | RuvC |
| TracrRNA requirement | No |
| PAM/PFS | NGG |
| Insertion | Csn |
| Construction | YFP-CBA-CD79α-SaCas9-ssRNA-Csn |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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