This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-CMV+intron-Sec/SPI-CD79α-CD8-Myc) and CRISPR-Cas (Cas13b (C2c4)-ssRNA-RuvC, HNH-NNGRRT-Wap) systems.
| Clone | TII11 |
| Host | Human |
| Targeting Cell Type | DS-1 |
| Reporter gene | GFP |
| Promoter | CMV |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | Myc |
| Linkers | Glycine linker |
| Co-Receptors | CD22 |
| Vector Type | Adenoviruses |
| Vector Name | BGpCDH3 |
| Class | 2 single-Cas |
| Type | III |
| Subtype | D |
| Effector | Cas13b (C2c4) |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | NNGRRT |
| Insertion | Wap |
| Construction | GFP-CMV-CD79α-Cas13b (C2c4)-ssRNA-Wap |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.