This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-hPGK-Sec/SPIII-CD79α-CD28-Flag) and CRISPR-Cas (Cas13a (C2c2)-RNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | BGPYH-1998 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | CAT |
| Promoter | hPGK |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Flag |
| Linkers | Glycine-Serine linker |
| Co-Receptors | CD22 |
| Vector Type | MMLV |
| Vector Name | BGpCDH5 |
| Class | 1 multi-Cas |
| Type | I |
| Subtype | A, B |
| Effector | Cas13a (C2c2) |
| DNA Type | RNA |
| Nuclease domains | HD/Cas3 |
| TracrRNA requirement | No |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | CAT-hPGK-CD79α-Cas13a (C2c2)-RNA-Stat5b |
| Target | HSD17B10 |
| Alternative Names | HSD17B10; 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1; hydroxysteroid (17-beta) dehydrogenase 10; hydroxysteroid 17-beta dehydrogenase 10; HSD10MD |
| Gene ID | 3028(Human); 15108(Mouse); 63864(Rat) |
| UniProt ID | Q16671(Human); Q8K592(Mouse); Q62893(Rat) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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