This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-CMV-Tat/SPI-CD79β-CD28-CBP) and CRISPR-Cas (NmCas9-ssRNA-RuvC, HNH-NGG-Csn) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | LAUL5 |
| Host | Rat |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | RFP |
| Promoter | CMV |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | CBP |
| Linkers | G4S linker |
| Co-Receptors | CD81 |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 3 double-Cas |
| Type | I |
| Subtype | A, B, C, D, E, F |
| Effector | NmCas9 |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | YES |
| PAM/PFS | NGG |
| Insertion | Csn |
| Construction | RFP-CMV-CD79β-NmCas9-ssRNA-Csn |
| Target | IL3RA |
| Alternative Names | IL3RA; Interleukin-3 receptor subunit alpha |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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