This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-EF1A-Sec/SPIII-CD79α-CD28-MBP) and CRISPR-Cas (Cas13a (C2c2)-ssRNA-RuvC, Nuc-NGG-Csn) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | X-5 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | RFP |
| Promoter | EF1A |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | MBP |
| Linkers | Glycine-Serine linker |
| Co-Receptors | CD81 |
| Vector Type | Adenoviruses |
| Vector Name | BGpCDH3 |
| Class | 1 multi-Cas |
| Type | III |
| Subtype | A, B |
| Effector | Cas13a (C2c2) |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, Nuc |
| TracrRNA requirement | Yes |
| PAM/PFS | NGG |
| Insertion | Csn |
| Construction | RFP-EF1A-CD79α-Cas13a (C2c2)-ssRNA-Csn |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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