This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (lacZ-CMV-Tat/SPI-CD79α-CD28-Flag) and CRISPR-Cas (Cas12b (C2c1)-RNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | LPM-2005 |
| Host | Mouse |
| Targeting Cell Type | RPMI 8226 |
| Reporter gene | lacZ |
| Promoter | CMV |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Flag |
| Linkers | Short Fusion Protein Linker |
| Co-Receptors | CD22 |
| Vector Type | Lentivirus |
| Vector Name | BGpCDH2 |
| Class | 2 single-Cas |
| Type | II |
| Subtype | A, B, C, D, E |
| Effector | Cas12b (C2c1) |
| DNA Type | RNA |
| Nuclease domains | HD/Cas3 |
| TracrRNA requirement | No |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | lacZ-CMV-CD79α-Cas12b (C2c1)-RNA-Stat5b |
| Target | MEIS2 |
| Alternative Names | Meis Homeobox 2; Meis1-Related Protein 1; MRG1; Meis1; Myeloid Ecotropic Viral Integration Site 1 Homolog 2 (Mouse); Meis1; Myeloid Ecotropic Viral Integration Site 1 Homolog 2; TALE Homeobox Protein Meis2; Meis (Mouse) Homolog 2; |
| Gene ID | 4212(Human); 17536(Mouse); 311311(Rat) |
| UniProt ID | O14770(Human); P97367(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.