This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-hPGK-Sec/SPIII-CD79β-CD28-Flag) and CRISPR-Cas (Cas12c (C2c3)-RNA-2xHEPN-NNNNGATT-Alb) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | 6i20 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | GUS |
| Promoter | hPGK |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | Flag |
| Linkers | Flexible Linker |
| Co-Receptors | CD19 |
| Vector Type | MMLV |
| Vector Name | BGpCDH5 |
| Class | 3 double-Cas |
| Type | II |
| Subtype | A, B, C, D, E, F |
| Effector | Cas12c (C2c3) |
| DNA Type | RNA |
| Nuclease domains | 2xHEPN |
| TracrRNA requirement | YES |
| PAM/PFS | NNNNGATT |
| Insertion | Alb |
| Construction | GUS-hPGK-CD79β-Cas12c (C2c3)-RNA-Alb |
| Target | MET |
| Alternative Names | MET Proto-Oncogene, Receptor Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1 |
| Gene ID | 4233(Human); 17295(Mouse); 24553(Rat) |
| UniProt ID | P08581(Human); P16056(Mouse); P97523(Rat) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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