This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-CMV+intron-Sec/SPI-CD79β-CD8-GST) and CRISPR-Cas (Cas12a (Cpf1)-ssRNA-RuvC, HNH-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | D1P-7YB7Y |
| Host | Human |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | GUS |
| Promoter | CMV+intron |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79β |
| TM domains | CD8 |
| Detection tags | GST |
| Linkers | GSAT Linker |
| Co-Receptors | CD72 |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 1 multi-Cas |
| Type | VI |
| Subtype | A, B, C, D, E, F |
| Effector | Cas12a (Cpf1) |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | 5' AT-rich PAM |
| Insertion | ROSA26 |
| Construction | GUS-CMV+intron-CD79β-Cas12a (Cpf1)-ssRNA-ROSA26 |
| Target | MET |
| Alternative Names | MET Proto-Oncogene, Receptor Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1 |
| Gene ID | 4233(Human); 17295(Mouse); 24553(Rat) |
| UniProt ID | P08581(Human); P16056(Mouse); P97523(Rat) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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