This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPIII-CD79α-CD8-CBP) and CRISPR-Cas (SaCas9-ssRNA-2xHEPN-NNNNGATT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | MvDN60 |
| Host | Humanized |
| Targeting Cell Type | Jiyoye [Jijoye, P-2003, P-3J] |
| Reporter gene | RFP |
| Promoter | mPGK |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | CBP |
| Linkers | Z-EGFR-1907 Linker |
| Co-Receptors | FcγRIIB |
| Vector Type | RABV |
| Vector Name | BGpCDH8 |
| Class | 2 single-Cas |
| Type | III |
| Subtype | A, B, C, D, E, F, U |
| Effector | SaCas9 |
| DNA Type | ssRNA |
| Nuclease domains | 2xHEPN |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | Wap |
| Construction | RFP-mPGK-CD79α-SaCas9-ssRNA-Wap |
| Target | MET |
| Alternative Names | MET Proto-Oncogene, Receptor Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1 |
| Gene ID | 4233(Human); 17295(Mouse); 24553(Rat) |
| UniProt ID | P08581(Human); P16056(Mouse); P97523(Rat) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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