This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-mPGK-Sec/SPIII-CD79α-CD8-GST) and CRISPR-Cas (Cascade-dsDNA-RuvC, HNH-NGG-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | T.7Y07Y.5 |
| Host | Mouse |
| Targeting Cell Type | SU-DHL-8 |
| Reporter gene | Luc |
| Promoter | mPGK |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | GST |
| Linkers | Short Fusion Protein Linker |
| Co-Receptors | Siglec-G |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 2 single-Cas |
| Type | III |
| Subtype | A, B, C, D, E |
| Effector | Cascade |
| DNA Type | dsDNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | NGG |
| Insertion | attP |
| Construction | Luc-mPGK-CD79α-Cascade-dsDNA-attP |
| Target | MET |
| Alternative Names | MET Proto-Oncogene, Receptor Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1 |
| Gene ID | 4233(Human); 17295(Mouse); 24553(Rat) |
| UniProt ID | P08581(Human); P16056(Mouse); P97523(Rat) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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