This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-CBh-Tat/SPI-CD79α-CD28-Myc) and CRISPR-Cas (NmCas9-ssRNA-HD/Cas10-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | BIWJN-067Y5 |
| Host | Mouse |
| Targeting Cell Type | CA46 |
| Reporter gene | GFP |
| Promoter | CBh |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | G4S linker |
| Co-Receptors | LILRB |
| Vector Type | MSCV |
| Vector Name | BGpCDH6 |
| Class | 1 multi-Cas |
| Type | IV |
| Subtype | A, B, C |
| Effector | NmCas9 |
| DNA Type | ssRNA |
| Nuclease domains | HD/Cas10 |
| TracrRNA requirement | No |
| PAM/PFS | 5' AT-rich PAM |
| Insertion | ROSA26 |
| Construction | GFP-CBh-CD79α-NmCas9-ssRNA-ROSA26 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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